The Indian wolf is an endangered species and evolutionary significant unit endemic to the Indian sub-continent. While they have a fragmented distribution across the native range, Gujarat is considered a bastion for the species with the contiguous Saurashtra-Kachchh-Thar landscape reported to hold the largest potential for wolf occupancy in India. Wolves were reported to occur in the Jamnagar district of the Saurashtra region about four decades ago, and persist to this day. Here we discuss the occurrence and conservation threats based on observations made during 2022 – 2024 on two wolf packs consisting of seven and four individuals each near the Gulf of Kachchh in Jamnagar. The wolves occupied approximately 80 km2, of which protected areas constitute about 39%. The wolves seem to have persisted in the region owing to the relatively unchanged land use pattern and increased vegetation cover in these areas signifying the ecological role of such refugia. However, the non- protected areas within the wolf-occupied region are undergoing rampant and unprecedented land use change which poses a grave threat to the wolf habitat and movement corridors. Such anthropogenic pressure on the wolf habitat would also ulti- mately affect the wild prey populations inhabiting the region. Additionally, human-wolf conflict, hybridisation with domestic dogs, as well as diseases, all pose threats to this wolf population. The persistence of wolves may not hold strong if the current circumstances are allowed to prevail. The current situation warrants devising policy decisions aimed at wolf conservation planning on an urgent basis. Since the study region is a multi-use landscape with multiple stakeholders involved, a suitable management approach that incorporates social, economic, and ecological dimensions can ensure the long-term persistence of the wolf population in the region.